SCOPE™
Protein Fractionation That Preserves Function
and Complex Information
SCOPE™ (Solvation Controlled Protein Enrichment) is a a groundbreaking protein fractionation technology that maintains both protein functionality and the integrity of protein complexes. SCOPE™ represents a transformative advance in proteomics, offering unprecedented insight into cellular mechanisms and disease pathways.
Traditional sample preparation techniques for proteomics using mass spectrometry often compromise the structural and functional integrity of proteins and their associated complexes, limiting researchers’ ability to analyze native interactions and biological activity. SCOPE™ overcomes these limitations, enabling scientists to fractionate proteins rapidly in a way that preserves their natural state— both as individual molecules and as part of larger, biologically relevant complexes.
“With SCOPE™, we’re opening a new window into proteomics which we call ‘the living proteome’,” said Prof David Hornby, CSO and co-founder at Entropix. “For the first time, researchers can isolate proteins without losing the context of their functional networks. This is a critical step in understanding how diseases like cancer, neurodegeneration, and autoimmune disorders disrupt cellular communication. SCOPE™ builds on our earlier success with NADA™ technology for nucleic acid capture and recovery.”
As shown in the SDS-PAGE gel image of the yeast proteome, SCOPE™ produces a rapid, highly controlled fractionation by precipitation without denaturation (on the left). The precipitated proteins can be re-solvated easily using a second reagent called RESOLVE™ (shown on the right).
These fractions can be analysed directly using mass spectrometry (MS) and cryo Electron Microscopy (EM). Each fraction contains intact protein complexes as revealed in our MS analysis. Similar results have been obtained from human/mammalian cells, plant cells and bacteria.
The SCOPE-RESOLVE™ technology could also offer benefits for the rapid purification of plant, animal or fermented proteins for food applications.
Key Features of SCOPE™:
- Rapid Capture of Protein: minimises sample degradation without denaturation.
- Preservation of Protein Function: SCOPE™ utilizes optimized conditions that retain enzymatic and signaling activity, allowing direct analysis of protein function post-fractionation.
- Maintenance of Protein Complexes: The technology protects native protein-protein interactions, enabling researchers to study stable and transient complexes in their biologically relevant forms including post-translational modifications.
- Versatile Application: Protein fractions created using SCOPE™ can be used directly in downstream analytical workflows for MS, EM, structural biology, and drug discovery.
This innovative approach has already demonstrated powerful utility in uncovering novel interactions in disease models and identifying therapeutic targets that would have been missed using conventional methods.
SCOPE™ is now available for research use, with early access opportunities for academic, clinical, and industry collaborators. For more information, technical specifications, and partnership inquiries, please contact CEO Rob Rule at enquires@entropix.co.uk.